Masterclass Webinar: Challenging Multiplex IHC Image Analysis in HALO®

Date: 23 February 2023

Time: 8:00 – 9:00 PST | 11:00 – 12:00 EST | 16:00 – 17:00 GMT

Location: Webinar

Learn advanced multiplex IHC workflows and how to manage challenging multiplex assays


In this 60-minute webinar, we discuss best practices and optimization techniques for advanced quantitative multiplex immunohistochemical (mIHC) image analysis. First, we will present a series of mIHC assays where various chromogen combinations were tested on an assay comprised of three biomarkers and hematoxylin. From this dataset, we discuss which assays are appropriate for image analysis with our HALO® Multiplex IHC module, which are not, and why.
Two alternative approaches for analyzing mIHC will also be demonstrated including a serial stain workflow to convert brightfield images into pseudo-fluorescence images followed by analysis with Highplex FL and a HALO AI-based approach where cell phenotyping is used to identify cells with co-localization of specific stains. We will discuss advantages, disadvantages and use cases for each workflow.

Learning Objectives

  • Advance your brightfield image deconvolution for mIHC analysis with tips and tricks
  • See examples of successful 3-plex chromogenic image analysis as well as images that are problematic and learn from common challenges
  • Learn how to perform a serial stain image analysis workflow to convert multiplexed immunohistochemistry to pseudo-flourescent images.  
  • Learn advantages of AI-based phenotyping and how to perform it with HALO AI


Dr. Kate Lillard Tunstall
Chief Scientific Officer
Indica Labs

Kate Lillard received her Ph.D. in Molecular Genetics and Biochemistry from the University of Cincinnati Medical Center, followed by a Howard Hughes postdoctoral fellowship at the University of Texas Southwestern Medical Center. While conducting research in the area of stem cell biology and oncology as a graduate and postdoctoral fellow, Dr. Lillard developed a keen interest in IHC which led to her joining Aperio in 2007 where she supported and then managed image analysis products for digital pathology. After acquisition of Aperio by Leica in late 2012, Dr. Lillard joined Indica Labs as Chief Scientific Officer where she supports, promotes, and helps guide the development of digital pathology image analysis solutions for the life sciences.

Source: Indica Labs

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