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SUMMARY:Masterclass: Unlock Cell Population Discovery Using HALO® High Dimensional Analysis
DESCRIPTION:0000Days00Hrs00Min00SecThe webinar starts at 7:00 PDT\, 10:00 EDT\, 15:00 BST \nLearn how unbiased high-dimensional analysis can be seamlessly combined with AI-powered HALO workflows for multiplex images!\n\n\nSummary\n\n\n\n\nMultiplexed imaging generates rich datasets where extracting meaningful insights requires more than predefined gates.  \nIn this webinar\, we present a real-world use case analyzing tertiary lymphoid structures (TLS) in non-small cell lung cancer (NSCLC) using whole-slide multiplex immunofluorescence images acquired on the Orion™ platform (RareCyte). We demonstrate how standard HALO® workflows\, including AI-driven tissue classification and cell segmentation\, can be seamlessly combined with unbiased high-dimensional analysis\, all within the same software.  \nUsing K-means\, Phenograph\, and UMAP\, we explore phenotypic diversity within TLS from dense tumor regions and highly infiltrated zones. HALO® High Dimensional Analysis reveals biologically relevant differences between these zones beyond supervised phenotypes. Importantly\, the dynamic link between UMAP plots and the original tissue image provides biological meaning to clustering results and enables clear spatial validation.  \n\n\n\n\n\nLearning Objectives\n\n\n\n\n\nAssess sample cell composition “at a glance” using K-means and Phenograph. \nUse unsupervised analysis to quality control your analysis pipeline. \nNavigate interactively between the UMAP and biological image. \nApply an iterative\, data-driven strategy to identify and discover biologically relevant phenotypes. \n\n\n\n\nRegister Now\nPresenter\n\nDr. Ghislaine Lioux\nSenior Field Applications Scientist \nIndica Labs  \nDr. Ghislaine Lioux earned her PhD from the Spanish National Institute of Cardiovascular Research in Madrid as a Marie Curie Fellow\, where she studied molecular mechanisms of heart development. Ghislaine joined Indica Labs in 2020\, and in her current position as a Senior Field Applications Scientist she supports customers\, distributors\, and collaborators on the technical use of HALO software. 
URL:https://www.pathologynews.com/event/masterclass-unlock-cell-population-discovery-using-halo-high-dimensional-analysis/
LOCATION:Online
CATEGORIES:Live Online Webinar
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